The FLIPR assay was carried out on FDSS/µCell with ligand addition at ten?s to the binding assay and mAb1/isotype addition at 10?s accompanied by hCCL1 addition at 300?s with the Levels of competition binding assay, with a total checking of 500?s. The excitation and emission wavelengths were being set at 485?nm and 525?nm, respectively. Once the r… Read More